Coding

Part:BBa_K4716986

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


PEP-6his tag

PEP is also a prolyl endopeptidase gluten breaking enzyme which is derived from Aspergillus oryzae RIB40; It has been used as a study of oral enzyme therapy for consumption in celiac disease patients. And for this basic part we add a his-tag at the end of PEP sequence to detect the expression of PEP enzyme protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 993
    Illegal XhoI site found at 1756
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1114


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Categories
Parameters
None